Fig. 6.

MM tumor development is significantly reduced in vivo in clodronate-liposome–treated mice.

KaLwRij mice were inoculated i.v. with 5TGM1 MM tumor cells, and tumor was allowed to establish for 2 weeks, followed by a single treatment with PBS-lip or clo-lip (n = 7/group). (A) Tumor burden was measured by BLI at 2, 3, and 4 weeks post tumor cell inoculation. Graph shows mean ± SEM, ***P < .001, two-way ANOVA with Sidak's multiple comparisons test. (B) Representative BLI images for each treatment group at week 4. (C) Serum paraprotein quantitation 4 weeks post tumor cell inoculation. (D) Flow cytometric analysis of GFP⁺ 5TGM1 within the peripheral circulation at 4 weeks post tumor cell inoculation. Graphs show mean ± SEM; *P < .05, unpaired t test.