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. 2019 Jun 22;21(8):765–776. doi: 10.1016/j.neo.2019.05.007

Figure 2.

Figure 2

High-throughput miRNA expression profiling in SOX4 knockdown PCa cells. (A) The protein expression levels of SOX4 in human PCa cell lines determined by Western blot. (B) VCaP and LNCaP cells with silencing SOX4 expression as well as DU145 and PC3 cells with SOX4 overexpression were established by transiently transfection in 48 hours. Levels of SOX4 knockdown and overexpression were quantified and normalized to GAPDH levels. (C) Heatmap show the top 25 downregulated miRNAs and top 20 upregulated miRNAs by siSOX4 in VCaP cells. (D) A Venn diagram depicting seven miRNAs that meet the following properties: (a) >2.0-fold change miRNAs downregulated by siSOX4 in VCaP; (b) top 100 miRNAs Pearson correlated with SOX4 in TCGA; (c) >2.0-fold change miRNAs upregulated in castration-resistant xenograft tissue compared to androgen-dependent xenograft tissue from GSE55829. (E) miR17-92 cluster members located within the same chromosome. (F) miR-19a and miR-19b downregulated genes are enriched for upregulation upon SOX4 knockdown from GSE11914.