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. 2019 Jun 22;21(8):765–776. doi: 10.1016/j.neo.2019.05.007

Figure 7.

Figure 7

SOX4 knockdown restrains neuroendocrine differentiation and proliferation of LNCaP-NEPC cells. (A) Analysis of the protein expression of RB1, TP53, and E2F1 in SOX4 knockdown/overexpression PCa cells detected by Western blot. (B) The morphology of LNCaP and LNCaP-NEPC cells was shown in the top. LNCaP-NEPC was established by continuously culturing the LNCaP cells in medium with 10% FBS depleted of steroids for 3 months. As shown in bottom, NEPC markers and SOX4 expression were detected by Western blot and RT- qPCR. (C) LNCaP-NEPC cells were infected with shNC or shSOX4 lentivirus and selected for stable cells. The morphology of the control and SOX4 knockdown cells was shown on the left. Shown on the right is the percentage of cells with epithelial and neuroendocrine-like phenotype. (D) Cell proliferation was examined by MTS. *P<.05. P values based on Student’s t test. (E) The expression of NEPC markers and SOX4 in shNC and shSOX4 LNCaP-NEPC cells was measured by Western blot and RT-qPCR. (F) A model depicting SOX4, downregulating RB1 protein via upregulating miR-17-92 cluster, promotes PCa progression.