TABLE 2.
Strains and plasmid used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| K. kingae strains | ||
| KK01 | Nonspreading/noncorroding derivative of 269-492 | 5 |
| KK01 csaA | KK01 with csaA deletion | 12 |
| KK01 pam | KK01 with pamABCDE deletion | 11 |
| KK01 csaA pam | KK01 with csaA deletion and pamABCDE deletion | 10 |
| KK01 rtx | KK01 with rtxBDCA deletion | This study |
| KK01 csaA rtx | KK01 with csaA deletion and rtxBDCA deletion | This study |
| KK01 pam rtx | KK01 with pam deletion and rtxBDCA deletion | This study |
| KK01 csaA pam rtx | KK01 with csaA deletion, pam deletion, and rtxBDCA deletion | This study |
| E. coli strain | ||
| DH5α | λ− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | 47 |
| Plasmid | ||
| pUC19rtxBDCA::kan | pUC19 with aphA3 kanamycin cassette flanked by surrounding 5′ and 3′ regions of the rtxBDCA locus | This study |