FIG 2.

Labeling specificity of SLE in live-cell imaging. HeLa cells stably expressing LAMP1-meGFP were seeded in 8-well chamber slides. The next day, cells were infected with STM ΔpipB2 harboring plasmids for expression of pipB2::HaloTag::HA, pipB2::SNAP-tag::HA, or pipB2::CLIP-tag::HA at an MOI of 50. Live-cell imaging was performed at 16 h postinfection. Labeling reactions were performed directly before imaging using the respective SLE ligand for 30 min at 37°C. Representative STM-infected host cells exhibiting SIF formation were selected for live-cell imaging. Scale bars, 10 μm.