FIG 5.

ΔhupY mutants are impaired in growth with serum or hemoglobin as the sole iron source. Shown is growth (20 h) of NZ131 wild-type (blue), ΔhupY mutant (red), and complemented (ΔhupY/pLC007 [gray]) strains in THYB, THYB with dipyridyl (DP), or THYB-DP supplemented with human serum (5 to 20% final volume) (A) or human hemoglobin (B). The data are from two independent experiments done in technical triplicates, with SD shown. The asterisk indicates significance (P < 0.05, Student’s t test, equal variance).