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. 2019 Jun 25;10(3):e00850-19. doi: 10.1128/mBio.00850-19

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Copyright © 2019 Fay et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — MsTtfA and MtbTtfA are required for TMM transport. (A) Thin-layer chromatographs (TLCs) of extractable mycolic acids from three replicate ¹⁴C-acetic acid-labeled M. smegmatis cultures carrying CRISPRi targeting guide RNAs (nontargeting [MGM6418]; left), ttfA (MGM6419; middle), or mmpL3 (MGM6637; right). Isoniazid treatment served as a positive control for inhibition of all mycolate synthesis. (B) Graph of TDM/TMM ratios for quantitation of TMM and TDM of TLCs in panel A. (C) TLCs of extractable mycolic acids from three replicate ¹⁴C-acetic acid-labeled M. tuberculosis cultures depleted for TtfA (MGM6675; left) or MmpL3 (MGM6676; right) (D) Quantitation of TDM/TMM ratios from quantitation of TMM and TDM of TLCs in panel C. ***, P < 0.01.