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MSMEG_5308 localizes to cell poles and septa and stabilizes the TtfA/MmpL3 interaction. (A) MSMEG_5308-msfGFP expression strain (MGM6681) imaged during logarithmic growth. GFP, DIC, and merged images are shown. (B) GFP-Trap pulldown of TtfA-msfGFP/MmpL3-mCherry coexpression strains with nontargeting or MSMEG_5308-targeting CRISPRi constructs. Inputs (left) and eluates (right) from the GFP-Trap column in the presence of either DDM or Triton X-100.
