TABLE 1.
S. coelicolor glycoproteins identified in this work
| Protein | Function | TMHMM no.a | SignalP 4.1b | TatP 1.0c | LipoP 1.0d | Classification |
|---|---|---|---|---|---|---|
| SCO0472 | Putative secreted protein | Y; 0.548 | Y; 0.381 | SpII; 22.2623 | Lipoprotein | |
| SCO0996 | Putative metal-binding lipoprotein | Y; 0.526 | N | SpI; 11.5964 | Lipoprotein | |
| SCO1714 | Putative secreted protein | 1 | Y; 0.498 | N | SpII; 12.878 | Lipoprotein |
| SCO2838 | Putative secreted endoglucanase | Y; 0.639 | Y; 0.377 | SpII; 32.6736 | Lipoprotein | |
| SCO3357 | Hypothetical protein | N | Y; 0.492 | SpII; 17.3077 | Lipoprotein | |
| SCO4142 | PstS, substrate binding domain of ABC-type phosphate transporter | Y; 0.595 | N | SpII; 26.7983 | Lipoprotein | |
| SCO4739 | Putative lipoprotein | Y; 0.579 | N | SpII; 20.7928 | Lipoprotein | |
| SCO4885 | Putative nucleoside-binding lipoprotein | N | N | SpII; 23.8395 | Lipoprotein | |
| SCO4905 | Putative lipoprotein | Y; 0.574 | N | SpII; 13.7291 | Lipoprotein | |
| SCO4934 | Putative l,d-transpeptidase | Y; 0.571 | Y; 0.483 | SpII; 24.1553 | Lipoprotein | |
| SCO5646 | Putative thiamine-binding lipoprotein | N | Y; 0.468 | SpII; 13.5061 | Lipoprotein | |
| SCO7218 | Putative iron transport lipoprotein | Y; 0.632 | N | SpI; 14.1761 | Lipoprotein | |
| SCO2096 | Transglutaminase/protease-like membrane protein | 6 | Y; 0.529 | N | SpII; 8.2333 | Membrane |
| SCO2035 | Putative disulfide oxidoreductase | 1 | N | N | N | Membrane |
| SCO2156 | Putative cytochrome c oxidase subunit II | 3 | N | N | N | Membrane |
| SCO2963 | Putative membrane protein | 1 | N | N | N | Membrane |
| SCO3044 | Putative cell envelope-associated transcriptional attenuator LytR-CpsA-Psr | 1 | N | N | N | Membrane |
| SCO3046 | Putative cell envelope-associated transcriptional attenuator LytR-CpsA-Psr | 1 | N | N | N | Membrane |
| SCO3184 | Putative penicillin acylase | 1 | N | Y; 0.366 | N | Membrane |
| SCO3848 | Putative serine/threonine protein kinase | 1 | N | N | N | Membrane |
| SCO3891 | Putative membrane protein | 1 | N | N | N | Membrane |
| SCO4013 | Putative secreted penicillin-binding protein FtsI | 1 | N | N | N | Membrane |
| SCO4130 | Putative integral membrane protein | 1 | N | N | N | Membrane |
| SCO4141 | Phosphate ABC transport system permease protein | 5 | N | N | N | Membrane |
| SCO4256 | Putative hydrolytic protein | 1 | N | N | N | Membrane |
| SCO4548 | Putative integral membrane protein | 3 | N | Y; 0.479 | N | Membrane |
| SCO4968 | Putative membrane protein | 1 | N | N | N | Membrane |
| SCO5204 | Integral membrane protein | 7 | N | N | N | Membrane |
| SCO5751 | Putative membrane protein | 1 | N | N | N | Membrane |
| SCO5818 | Putative ABC-type Na+ transport system | 5 | N | N | N | Membrane |
| SCO3540 | Proteinase (putative secreted protein) | 1 | Y; 0.627 | Y; 0.700 | SpI; 18.2099 | Secreted |
| SCO4847 | DacC, putative d-alanyl-d-alanine carboxypeptidase | 1 | Y; 0.711 | Y; 0.427 | SpI; 27.3476 | Secreted |
| SCO5776 | Glutamate binding protein | Y; 0.618 | N | SpI; 21.8509 | Secreted | |
| SCO3353 | Hypothetical protein | N | N | N | Other | |
| SCO4307 | MurQ, N-acetylmuramic acid-6-phosphate etherase | N | N | N | Other | |
| SCO5115 | BldKD, putative ABC transporter intracellular ATPase subunit | N | N | N | Other | |
| SCO5736 | 30S ribosomal protein S15 | N | N | N | Other | |
| SCO6558 | Putative oxidoreductase | N | N | N | Other |
a
The number of transmembrane helices predicted by the TMHMM 2.0 server (http://www.cbs.dtu.dk/services/TMHMM/).
b
SignalP 4.1 software predicts the presence of a signal peptide (http://www.cbs.dtu.dk/services/SignalP/). d-score is a score used to discriminate signal peptides from non-signal peptides. Scores of >0.450 indicate a signal peptide. Y, yes; N, no.
c
TatP 1.0 predicts the presence of twin arginine (TAT) signal peptides. d-score of >0.36 predicts the presence of a TAT pathway signal.
d
LipoP 1.0 software produces predictions of lipoproteins (http://www.cbs.dtu.dk/services/LipoP/). SpI denotes SEC signal peptide; SpII denotes lipoprotein.