FIG 5.

SaeP-induced biofilm enhancement is dependent on eDNA. (A) SaeP was expressed from pEPSA5 in an atlA mutant under biofilm-forming conditions using a range of xylose concentrations (0 to 0.6%) to induce protein expression. Error bars are the standard deviation for three replicate wells. (B) SaeP expression results in the accumulation of eDNA in the biofilm matrix. Static biofilms expressing either SaeP or DsbA were grown in TSB supplemented with 0.4% glucose and xylose at the indicated concentrations. eDNA isolated from the biofilm matrix was run on agarose gels. The numbers to the left of the gel are MW (in kilodaltons). (C) Biofilm enhancement of LAC expressing WT SaeP at 0.6% xylose is reduced when purified Nuc is added at time zero. Statistics for two-way analysis of variance are P values. *, P ≤ 0.05; **, P ≤ 0.005; ***, P ≤ 0.0005; ****, P ≤ 0.00005.