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. 2019 Jun 25;10(3):e01137-19. doi: 10.1128/mBio.01137-19

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Copyright © 2019 Kavanaugh et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — Lipoprotein mutants show increased biofilm porosity. (A) Biofilm biomass for Csa knockout mutants measured by crystal violet staining and expressed as a fraction of the WT biomass. Values are averages from either two or three experiments (>45 wells per strain tested). (B) Concentrations of different-molecular-weight FITC-dextrans in the flowthrough of filter-grown biofilms (12 wells per experiment). Concentrations are expressed as the fraction of the FITC-dextran concentration in the flowthrough of wells containing sterile medium (i.e., no-biofilm control wells). Statistics for two-way analysis of variance are P values. *, P ≤ 0.05; **, P ≤ 0.005; ***, P ≤ 0.0005. (C) Protein band intensities from SDS-PAGE of the filter-grown biofilm flowthrough, expressed as the fraction of the intensity for the corresponding band in the protein loading solution. Values are the averages from two experiments with >14 wells per strain tested (except that 3 wells were used for the triple mutant). Statistics for two-way analysis of variance are P values. *, P ≤ 0.05; **, P ≤ 0.005. (D) Biofilm biomass for the WT and CopL mutant measured by crystal violet staining and expressed as a fraction of the WT biomass. Values are averages from two experiments with >40 wells per strain tested. Statistics for one-way analysis of variance are P values. *, P ≤ 0.05; **, P ≤ 0.005. (E) Concentrations of different-molecular-weight FITC-dextrans in the flowthrough of filter-grown biofilms. Concentrations are expressed as the fraction of the FITC-dextran concentration in the flowthrough of wells containing sterile medium. Values are the averages from two experiments with 12 wells per strain per experiment. Statistics for two-way analysis of variance are P values. **, P ≤ 0.005.