FIG 6.
Neutrophils are partially responsible for vCXCL-1Tol-induced inflammation and dissemination. (A) Neutrophil depletion experimental design. αLy6G, anti-Ly6G antibody; FP, footpad; LN, lymph node. (B) Footpad swelling of infected footpads up to 3 dpi normalized to uninfected footpads. (C) Plaque assays were performed on LN harvested from depleted or control mice at 3 dpi. Values are averages of two experiments with three mice per group. Bars represent the average titers ± SD (error bars). Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparison of means and indicated as follows: ***, P ≤ 0.001; ****, P ≤ 0.0001.