Table 1.

Amino acid residues chosen for mutation based on a combination of elevated B‐factors and the proline rule.

Mutation	Secondary structure element	B‐factor [Å2]
K69P	α‐helix 3	28.1
K167P	α‐helix 7	25.5
K193P	β‐turn 8	38.5
D218P	β‐turn 9	23.1
K304P	α‐helix 11	22.1
E368P	β‐turn 16	39.0
G398P	α‐helix 15	28.3
R432P	α‐helix 16	26.9