Table 2.
Kinetic parameters of Cv‐ATA WT and all variants at 25 °C.
| Specific activity |
|
|
/ [b] | |||
|---|---|---|---|---|---|---|
| [U mg−1][a] | [mm][b] | [U mg−1][b] | ||||
| WT | 4.05 | 7.60 | 8.18 | 1.08 | ||
| Pro8 | n.d.[c] | – | – | – | ||
| K69P/K304P | 6.67 | 11.0 | 19.7 | 1.79 | ||
| K167P/E368P | 3.04 | 10.6 | 8.42 | 0.794 | ||
| K193P/G398P | n.d.[c] | – | – | – | ||
| D218P/R432P | 5.07 | 6.65 | 9.35 | 1.41 | ||
| K193P | 3.99 | 8.80 | 8.78 | 1.00 | ||
| G398P | n.d.[c] | – | – | – | ||
| Pro7 | 4.28 | 6.48 | 7.84 | 1.21 | ||
| K69P | 3.82 | 8.18 | 8.25 | 1.01 | ||
| K167P | 2.59 | 6.39 | 4.80 | 0.751 | ||
| D218P | 2.33 | 9.48 | 5.93 | 0.626 | ||
| K304P | 3.99 | 9.33 | 9.43 | 1.01 | ||
| E368P | 4.97 | 8.14 | 10.5 | 1.29 | ||
| R432P | 3.65 | 5.50 | 6.00 | 1.09 | ||
| Pro4 | 5.73 | 7.26 | 11.5 | 1.58 | ||
| Pro5 | 5.10 | 7.89 | 11.9 | 1.51 |
[a] Specific activity based on a model reaction with (S)‐1‐phenylethylamine and pyruvate where substrate concentrations are fixed at 2.5 mm. [b] Apparent kinetic constants were determined under pseudo‐one‐substrate conditions. [c] Not detected.