Figure 2.

TREM‐1 regulated inflammatory cytokines and lipid accumulation. qRT‐PCR analysis of TREM‐1 mRNA in stable cell lines of HepG2 and PMH treated with OA that either overexpression or knockdown of TREM‐1. Western blot analysis of TREM‐1 protein in stable cell lines of HepG2 and PMH treated with OA that either overexpression or knockdown of TREM‐1. qRT‐PCR analysis of IL‐1β, IL‐6, TNF‐α, IFN‐γ, MCP‐1, and MIP‐1α mRNA in stable cell lines of HepG2 and PMH treated with OA that either overexpression or knockdown of TREM‐1. Oil Red O staining of HepG2 and PMH cells treated with OA that either overexpression or knockdown of TREM‐1. qRT‐PCR analysis of MSR1, LDLR, ABCA1, ABCG1, NPC1/2, and STARD4 mRNA in stable cell lines of HepG2 and PMH treated with OA that either overexpression or knockdown of TREM‐1. **P < 0.01 and ***P < 0.001. ABCA1, ATP‐binding cassette transporter‐1; ABCG1, ATP‐binding cassette sub‐family G member 1; GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; HFD, high‐fat diet; IFN, interferon; IL, interleukin; LDLR, low‐density lipoprotein receptor; MCP‐1, monocyte chemoattractant protein‐1; MIP‐1α, macrophage inflammatory protein‐1α; mRNA, messenger RNA; MSR1, macrophage scavenger receptor 1; NAFLD, nonalcoholic fatty liver disease; NC, negative control; NCD, normal control diet; NPC1, Niemann‐Pick disease, type C1; NPC2, NPC intracellular cholesterol transporter 2; OA, oleic acid; PMH, primary mouse hepatocytes; qRT‐PCR, quantitative reverse‐transcription polymerase chain reaction; siNC, small interfering negative control; STARD4, StAR‐related lipid transfer protein 4; TNF‐α, tumor necrosis factor‐α; TREM‐1, triggering receptor expressed on myeloid cells‐1