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. 2019 Jun 19;10:1361. doi: 10.3389/fimmu.2019.01361

Figure 1.

Figure 1

Expression of receptors for IL-33 (ST2) and TSLP (TSLP-R/IL7R) on various human mast cells. Surface expression of TSLP-R (A), IL7R (B), and ST2 (C) on ROSA (A–F), CBMC (A–C), and HLMCs (A–C) was measured by flow cytometry. Representative histograms in which dotted lines represent the respective isotype controls are shown for different cell lines to the left, and quantification of the data is shown to the right (A–C). Mast cells were treated with 10 ng/ml IL-33, TSLP or a combination or both repeatedly for 4 days; thereafter, surface expression of TSLP-R (D), IL7R (E), and ST2 (F) was measured by flow cytometry. The median fluorescent intensity (MFI) of the receptors was normalized to the respective isotype control. Data shown were pooled from three independent experiments, n = 3–6.