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Histamine release induced by prolonged exposure to IL-33. ROSA cells (A,B) or CBMCs (C,D) were treated with 10 ng/ml IL-33, TSLP or a combination of both for 1 h (A,C) or treated repeatedly (every day) for 4 days (B,D). Thereafter, supernatants were collected, and histamine was measured. Data shown were pooled from 3 to 5 independent experiments, n = 6–10.
