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Effect of IL-33 on mast cell degranulation by FcεRI crosslinking. CBMCs were treated with 10 ng/ml IL-33 for 1 h (A,C) or repeatedly every day for 4 days (B,D); thereafter, they were stimulated various concentrations of anti-IgE. Degranulation was measured by the detection of surface CD63 with flow cytometry (A,B) or as histamine release (C,D). Data shown were pooled from 3 independent experiments, n = 3.
