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Decrease in mRNA expression of the FcεRI receptor by IL-33. CBMCs were repeatedly treated every day for 4 days with 10 ng/ml IL-33, TSLP or a combination of both. In addition, IL-4 was added on day 0. The mRNA expression level of the different subunits of the FcεRI receptor were quantified using qPCR (A–C). Expression was normalized to that of the housekeeping gene GAPDH and thereafter to the unstimulated control. Data shown were pooled from six independent experiments, n = 6.
