Table 1.

Strains and oligonucleotides.

Oligonucleotides used to construct the pknB mutant

AphaA3-F: CCAGCGAACCATTTGAG

AphaA3-R: GTTGCGGATGTACTTCAG

1: ATGCTGTGATTTTCGCTC

2: GACATCTAATCTTTTCTGAAGTACATCCGCAACGGATTCGATAACGTCCAGCA

3: ATAATCTTACCTATCACCTCAAATGGTTCGCTGGAGTACGACACAATCGTCGTC

4: CAAAAATCCGCTCCCGAA

5: TAAAGGAATGGGAACGAC

6: GACCAATTCAACGTGAGA

Positions of the oligonucleotides and of the PCR fragments