Table 2.
List of S. thermophilus proteins in which phosphorylated peptides have been identified.
| Protein short name | Description | YP Protein Number | New WP non-redundant protein number sequence number | S. thermophilus LMD9 old locus number | S. thermophilus LMD9 new locus number |
|---|---|---|---|---|---|
| Amino acid metabolism and transport | |||||
| LivG | ABC transport system ATP-binding protein | YP_819896.1 | WP_002949740.1 | STER_0401 | STER_RS01955 |
| GdhA | Glutamate dehydrogenase/leucine dehydrogenase | YP_819946.1 | WP_011680803.1 | STER_0467 | STER_RS02295 |
| GlnQ | Amino acid ABC transporter ATP-binding protein | YP_820506.1 | WP_011681207.1 | STER_1117 | STER_RS05525 |
| TrpD | Anthranilate phosphoribosyl transferase | YP_820900.1 | WP_011681519.1 | STER_1552 | STER_RS07630 |
| AroG1 | 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase | YP_821042.1 | WP_002947699.1 | STER_1703 | STER_RS08325 |
| GlnA | Glutamine synthetase | YP_821087.1 | WP_011681641.1 | STER_1751 | STER_RS08555 |
| Carbohydrate metabolism and transport | |||||
| Pgi | Glucose-6-phosphate isomerase | YP_820037.1 | WP_011680683.1 | STER_0241 | STER_RS01180 |
| Tkt | Transketolase | YP_819848.1 | WP_011680734.1 | STER_0349 | STER_RS01700 |
| CcpA | Catabolite control protein A | YP_820134.1 | WP_004197422.1 | STER_0679 | STER_RS03345 |
| Eno | Enolase | YP_820138.1 | WP_011680939.1 | STER_0684 | STER_RS03365 |
| GlgP | Glycogen/starch/alpha-glucan family phosphorylase | YP_820422.1 | WP_011681138.1 | STER_1016 | STER_RS05015 |
| GpmA | Phosphoglycerate mutase | YP_820559.1 | WP_011226119.1 | STER_1172 | STER_RS05785 |
| Pyk | Pyruvate kinase | YP_820550.1 | WP_011681242.1 | STER_1163 | STER_RS05740 |
| PgmA | Phosphoglucosamine mutase | YP_820614.1 | WP_011226155.1 | STER_1230 | STER_RS06065 |
| PtsI | Phosphoenolpyruvate protein phosphotransferase | YP_820626.1 | WP_011681297.1 | STER_1242 | STER_RS06130 |
| PtsH | Phosphocarrier protein HPr | YP_820627.1 | WP_002946351.1 | STER_1243 | STER_RS06135 |
| Ldh | L-lactate dehydrogenase | YP_820638.1 | WP_011226177.1 | STER_1257 | STER_RS06205 |
| GapA1 | Glyceraldehyde 3-phosphate dehydrogenase | YP_821096.1 | WP_011681649.1 | STER_1761 | STER_RS08615 |
| Fba | Fructose/tagatose biphosphate aldolase | YP_821189.1 | WP_011681711.1 | STER_1876 | STER_RS09185 |
| LacZ | Beta-galactosidase | YP_820735.1 | WP_011226267.1 | STER_1366 | STER_RS06725 |
| LacS | PTS sugar transporter subunit IIA | YP_820736.1 | WP_011681393.1 | STER_1367 | STER_RS06730 |
| GalM | Galactose mutarotase | YP_820737.1 | WP_011226269.1 | STER_1368 | STER_RS06735 |
| GalE | UDP-glucose 4-epimerase | YP_820738.1 | WP_011681394.1 | STER_1369 | STER_RS06740 |
| PpnK | NAD kinase | YP_820780.1 | WP_014608552.1 | STER_1422 | STER_RS06995 |
| RgpA | Glycosyltransferase family 1 | YP_820795.1 | WP_011681439.1 | STER_1437 | STER_RS07070 |
| AmyL | Alpha-amylase | YP_820851.1 | WP_011681482.1 | STER_1500 | STER_RS07380 |
| Pfl | Pyruvate formate lyase | YP_820965.1 | WP_011226473.1 | STER_1622 | STER_RS07960 |
| ScrA | Sucrose PTS transporter subunit EIIBCA | YP_821049.1 | WP_011681617.1 | STER_1710 | STER_RS08355 |
| GalU | UDP-glucose-pyrophosphorylase | YP_821134.1 | WP_002953557.1 | STER_1810 | STER_RS08845 |
| Cell cycle control | |||||
| GpsB | Cell division protein | YP_819787.1 | WP_011680702.1 | STER_0280 | STER_RS01360 |
| PabC | Transporter/YceG motif involved in septum cleavage | YP_819793.1 | WP_011680707.1 | STER_0288 | STER_RS01395 |
| FtsW | FtsW/RodA/SpoVE family cell cycle protein | YP_819997.1 | WP_002945261.1 | STER_0523 | STER_RS02565 |
| FtsA | Cell division protein | YP_820223.1 | WP_011225777.1 | STER_0776 | STER_RS03805 |
| FtsZ | Cell division GTPase | YP_820224.1 | WP_011225778.1 | STER_0777 | STER_RS03810 |
| SepF | Cell division protein | YP_820226.1 | WP_011681005.1 | STER_0779 | STER_RS03820 |
| DivIVA | Cell division initiation protein | YP_820229.1 | WP_011681008.1 | STER_0782 | |
| RodA | FtsW/RodA/SpoVE family cell cycle protein | YP_820583.1 | WP_011681262.1 | STER_1197 | STER_RS05905 |
| Cell wall/membrane/envelop biogenesis | |||||
| PcsB | CHAP domain-containing protein | YP_819611.1 | WP_011680584.1 | STER_0042 | STER_RS00210 |
| AcpP1 | Acyl carrier protein | YP_819616.1 | WP_002949008.1 | STER_0048 | STER_RS00245 |
| MurN | Aminoacyltransferase/Peptidoglycan interpeptide bridge formation enzyme | YP_820103.1 | WP_011680915.1 | STER_0644 | STER_RS03165 |
| RmlB | dTDP-D-glucose 4,6 dehydratase | YP_820607.1 | WP_002947383.1 | STER_1222 | STER_RS06025 |
| Energy production and conversion | |||||
| Adk | Adenylate kinase | YP_821196.1 | WP_011681712.1 | STER_1886 | STER_RS09240 |
| Inorganic ion transport and metabolism | |||||
| Pacl1 | Calcium translocating P-type ATPase, PMCA-type | YP_820251.1 | WP_011681025.1 | STER_0808 | STER_RS03975 |
| Cah | Carbonate dehydratase | YP_820966.1 | WP_011681555.1 | STER_1623 | STER_RS07965 |
| Intracellular trafficking and secretion | |||||
| YajC | Preprotein translocase YajC subunit | YP_819755.1 | WP_011225395.1 | STER_0243 | STER_RS01190 |
| SecA | Preprotein translocase SecA subunit | YP_821044.1 | WP_011681614.1 | STER_1705 | STER_RS08335 |
| Lipid metabolism | |||||
| AccB | Acetyl-CoA carboxylase, Biotin carboxyl carrier protein | YP_819919.1 | WP_011680783.1 | STER_0435 | STER_RS02130 |
| Nucleotide metabolism and transport | |||||
| ParE | DNA topoisomerase IV subunit B | YP_820096.1 | WP_011680910.1 | STER_0633 | STER_RS03105 |
| PyrE | Orotate phosphoribosyltransferase | YP_820393.1 | WP_011681117.1 | STER_0981 | STER_RS04840 |
| Apt | Adenine/guanine phosphoribosyltransferase | YP_820576.1 | WP_002947203.1 | STER_1190 | STER_RS05870 |
| LigA | NAD-dependent DNA ligase | YP_820862.1 | WP_023909862.1 | STER_1513 | STER_RS07445 |
| GuaB | IMP dehydrogenase/GMP reductase | YP_821293.1 | WP_011227627.1 | STER_1992 | STER_RS09740 |
| Post-translational modification, protein turnover, chaperone function | |||||
| GrpE | Nucleotide exchange factor/heat shock protein, chaperonin | YP_819701.1 | WP_011680648.1 | STER_0162 | STER_RS00785 |
| DnaK | Chaperonin | YP_819702.1 | WP_011226783.1 | STER_0163 | STER_RS00790 |
| Tig | Trigger factor | YP_819709.1 | WP_011680653.1 | STER_0191 | STER_RS00935 |
| GroES | Co-Chaperonin | YP_819764.1 | WP_002949332.1 | STER_0252 | STER_RS01225 |
| GroEL | Chaperonin | YP_819765.1 | WP_011225399.1 | STER_0253 | STER_RS01230 |
| PrsA | Foldase | YP_819969.1 | WP_011680821.1 | STER_0492 | STER_RS02415 |
| ClpE | ATP-dependent ClpP protease ATP-binding subunit | YP_820106.1 | WP_011680918.1 | STER_0648 | STER_RS03195 |
| TypA | Translational GTPase involved in stress response | YP_820218.1 | WP_011227074.1 | STER_0771 | STER_RS03780 |
| EpsD | Tyrosine protein kinase | YP_820463.1 | WP_011681174.1 | STER_1068 | STER_RS05305 |
| PknB | Ser/Thr kinase | YP_820752.1 | WP_011681404.1 | STER_1392 | STER_RS06845 |
| ClpL | ATP-dependent Clp protease ATP-binding subunit | YP_820924.1 | WP_011681535.1 | STER_1578 | STER_RS07755 |
| Asp23/Gls24 family envelope stress protein | YP_821053.1 | WP_002951891.1 | STER_1714 | STER_RS08375 | |
| Asp23/Gls24 family envelope stress protein | YP_821265.1 | WP_011681746.1 | STER_1962 | STER_RS03195 | |
| Translation | |||||
| RplU | 50S Ribosomal protein L21 | YP_819935.1 | WP_002885597.1 | STER_0455 | STER_RS02235 |
| PapL | CCA tRNA nucleotidyl transferase | YP_819940.1 | WP_011680798.1 | STER_0461 | STER_RS02265 |
| Frr | Ribosome recycling factor | YP_820913.1 | WP_002949868.1 | STER_0475 | STER_RS02335 |
| Tuf | Elongation factor | YP_819998.1 | WP_002949971.1 | STER_0524 | STER_RS02570 |
| RplL | 50S Ribosomal Protein L7/L12 | YP_820037.1 | WP_011680864.1 | STER_0568 | STER_RS02800 |
| RpsA | 30S Ribosomal protein S1 | YP_820100.1 | WP_011680912.1 | STER_0639 | STER_RS03135 |
| RpmE | 50S Ribosomal protein L31 type B | YP_820233.1 | WP_002945948.1 | STER_0787 | STER_RS03860 |
| PrfA | Peptide chain release factor A | YP_820239.1 | WP_002948472.1 | STER_0793 | STER_RS03890 |
| QueF | NADPH-dependent 7-cyano-7-deazaquanine reductase | YP_820305.1 | WP_002946191.1 | STER_ 0872 | STER_RS04310 |
| RpmC | 50S Ribosomal protein L29 | YP_821209.1 | WP_002952156.1 | STER_1899 | STER_RS09305 |
| Fus | Elongation factor G | YP_821097.1 | WP_011226574.1 | STER_1762 | STER_RS08620 |
| RpsF | 30S Ribosomal protein S6 | YP_821065.1 | WP_011681624.1 | STER_1728 | STER_RS08450 |
| RplA | 50S Ribosomal protein L1 | YP_821124.1 | WP_002946412.1 | STER_1797 | STER_RS08780 |
| Efp | Elongation factor P | YP_821054.1 | WP_011227545.1 | STER_1715 | STER_RS08380 |
| InfA | Translation initiation factor IF-1 | YP_821195.1 | WP_001040189.1 | STER_1885 | STER_RS09235 |
| RpmF | 50S Ribosomal protein L32 | WP_002952208.1 | STER_1953 | STER_RS09555 | |
| RpsD | 30S Ribosomal protein S4 | YP_821274.1 | WP_002952258.1 | STER_1973 | STER_RS09645 |
| RpoC | DNA-directed RNA polymerase beta subunit | YP_821165.1 | WP_011681690.1 | STER_1844 | STER_RS09010 |
| RplQ | 50S Ribosomal protein L17 | YP_821190.1 | WP_002952134.1 | STER_1880 | STER_RS09215 |
| RplR | 50S Ribosomal protein L18 | YP_821201.1 | WP_011681714.1 | STER_1891 | STER_RS09265 |
| RplF | 50S Ribosomal protein L6 | YP_821202.1 | WP_002946167.1 | STER_1892 | STER_RS09270 |
| RplE | 50S Ribosomal protein L5 | YP_821205.1 | WP_002887058.1 | STER_1895 | STER_RS09285 |
| RplV | 50S Ribosomal protein L22 | YP_821212.1 | WP_002887063.1 | STER_1902 | STER_RS09320 |
| RplB | 50S Ribosomal protein L2 | YP_821214.1 | WP_002952161.1 | STER_1904 | STER_RS09330 |
| Function unknown or putative | |||||
| Hypothetical protein | YP_819789.1 | WP_011680704.1 | STER_0283 | STER_RS01370 | |
| DNA-binding protein | YP_820441.1 | WP_002946488.1 | STER_0353 | STER_RS01720 | |
| Hypothetical | YP_819930.1 | WP_011680791.1 | STER_0449 | STER_RS02200 | |
| Hypothetical Sulfur transferase/rhodanese domain-containing protein | YP_820053.1 | WP_011680879.1 | STER_0588 | STER_RS02890 | |
| Hypothetical | YP_820069.1 | WP_011226984.1 | STER_0605 | STER_RS02975 | |
| HlyIII | Putative hemolysine III like | YP_820082.1 | WP_011680900.1 | STER_0618 | STER_RS03030 |
| LytR family transcriptional regulator | YP_820152.1 | WP_011680948.1 | STER_0698 | STER_RS03435 | |
| DUF948 domain-containing protein | YP_820173.1 | WP_011680965.1 | STER_0721 | STER_RS03545 | |
| Hypothetical/DUF3270 domain-containing protein | YP_820175.1 | WP_002945999.1 | STER_0723 | STER_RS03555 | |
| Hypothetical transpeptidase | YP_820213.1 | WP_011680998.1 | STER_0765 | STER_RS03750 | |
| BMP family ABC-transporter periplasmic substrate binding component | YP_820291.1 | WP_002950498.1 | STER_0856 | STER_RS04220 | |
| YufQ | ABC transport system, permease component | YP_820294.1 | WP_011681056.1 | STER_0859 | STER_RS04235 |
| HstH | HU family DNA-binding protein | YP_820562.1 | WP_002950996.1 | STER_1175 | STER_RS05800 |
| Hypothetical DegV protein | YP_820564.1 | WP_011681251.1 | STER_1178 | STER_RS05815 | |
| RpoC | Putative dihydroxyacetone kinase | YP_821175.1 | WP_011681697.1 | STER_1854 | STER_RS09055 |
| DUF965 domain-containing protein | YP_821243.1 | WP_011681731.1 | STER_1937 | STER_RS09475 | |
| XRE-family DNA-binding domain protein | YP_821288.1 | WP_011681760.1 | STER_1987 | STER_RS09715 | |
The proteins are classified in functional groups. The protein annotation was automatically performed as described in the Section “Materials and Methods.” The proteins in gray are those that have been identified as PknB targets (see Table 3).