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. 2019 Jun 25;16:14. doi: 10.1186/s12950-019-0219-x

Fig. 3.

Fig. 3

Chemokine expression in the kidneys of 18-month-old LEV rats. (a) The diagram shows the relative protein expression of eotaxin and MCP-1 in 1 ng of total kidney tissue extract from 18-month-old rats as the means ± SD of 8 CD-fed (open white bars) and 8 HFD-fed (solid black bars) male LEV rats. The mean eotaxin and MCP-1 levels in CD-fed rats were set to 1 for better comparison. Statistical significance (HFD vs. CD) was determined using two-way t-tests and is denoted by * for p ≤ 0.05. (b) The diagram shows the relative eotaxin and MCP-1 mRNA levels determined by QGP assay [MFI = mean fluorescence intensity] in kidneys from 18-month-old rats as the means ± SD of 8 CD-fed (open white bars) and 8 HFD-fed (solid black bars) male LEV rats. The differences between HFD- and CD-fed LEV rats were not significant as determined by two-way t-tests (p > 0.05)