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. 2019 Jun 26;20:525. doi: 10.1186/s12864-019-5906-8

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Outline of the feeding trial design during which RFI was measured. During each dietary stage, steers were offered the respective diet for 70 days following a period of dietary adaptation. At the end of each dietary stage, liver biopsies were taken and RFI was calculated. Within breed, all steers were ranked for RFI. RNA-Seq libraries were generated from biopsies taken from the most RFI-divergent steers (n = 12 high and n = 12 low)