Fig. 5.

I-CAM 1 ligand mediates the adhesion between feline ASCs and T lymphocytes. Fluorescent images of static adhesion assay demonstrating adherent lymphocytes to feline ASCs from a non-activated MLR, b stimulated MLR with ConA, c stimulated MLR with ConA and addition of ICAM-1 blocking antibodies, and d stimulated MLR with ConA and addition of ICAM-1/LFA-1 blocking antibodies. Scale bar = 400 μm. e Percentage of remaining fluorescence intensity from CMFDA-labeled PBMCs after removal of non-adherent cells from static adhesion assay with added condition of ICAM-1 blocking antibodies. Data is normalized to 100% on standard MLR condition for comparability. f Changes in IFN-γ concentration in the MLR after addition of ICAM-1 blocking antibodies. Data from 5 experiments, normalized to 100% on standard MLR condition for comparability. *p < 0.05, **p < 0.01