Fig. 4.

Orthotopic tumor growth is reduced by miR-221-5p overexpression in vivo. a Body weight was measured weekly along the course of the in vivo experiment (n = 4 for miR-221-5p and scrambled control). Data was analysed by two-way ANOVA repeated measures with Sidak’s multiple comparison test. b BLI imaging of mice injected with miR-221-5p or scrambled negative control. One representative image of each group is shown at 7 and 28 days. Tumor growth was quantified by overall counts per second (cps) from four animals per group (n = 4). The data was analysed by two-way ANOVA repeated measures with Sidak’s multiple comparison test. ** p < 0.01. c Tumor volume of miR-221-5p and scrambled overexpressing PC-3 M-Pro4luc2 cells was calculated as V = π/6 x length x width² and normalised to the body weight. Data was analysed by unpaired, two-tailed t-test. * p < 0.05. Scale bar = 5 mm. d H&E of scrambled and miR-221-5p group (left panel). The expression of proliferation marker PCNA (green; middle panel), apoptosis marker cleaved caspase-3 (cl. casp-3; red; right panel), tumor cell marker panCK (red; middle panel) and stroma marker α-SMA (green; right panel) was analysed by immunofluorescence staining