Table 1.
Bacterial strains and plasmids used in this study
| Plasmids | Characteristics | Reference |
| pValac | Eukaryotic expression vector (pCMV/Cmr/RepA/RepC) | [16] |
| pValac::anti-TNFα | pValac containing anti-TNFα scFv ORF | This study. |
| Bacterial strain and plasmids | Characteristics | Reference |
| Escherichia coli XL1-Blue | E. coli; F′[Tn10 proAB+ lacIq Δ (lacZ)M15] hsdR17(rK− mK+)/ Tetr | Stratagene® (Catalog no #200249) |
| Escherichia coli TG1 | E. coli, K-12-derived strain; F′ [traD36 proAB+ lacIq lacZΔM15] supE thi-1 Δ (lac-proAB) Δ (mcrB-hsdSM)5, (rK−mK−) | Lucigen, Middleton, MI, USA (Catalog no 60502–1) |
| Lactococcus lactis MG1363 FnBPA+ (LL-F) | L. lactis MG1363 strain expressing FnBPA of S. aureus (Eryr) | [15], obtained from Laboratory of Cellular and Molecular Genetics (LGCM), Federal University of Minas Gerais (UFMG), Brazil, |
| Lactococcus lactis MG1363 FnBPA+ (pValac::anti-TNFα) (LL-FT) | L. lactis MG1363 FnBPA+ strain carrying the pValac::anti-TNFα plasmid (Cmr/ Eryr) | This study. |
Cmr chloramphenicol resistance; Tetr tetracycline resistance; Eryr erythromicin resistance