Fig. 5.

Effect of MYOSLID on the invasion and metastasis of OSCC cell lines. a Effect of MYOSLID on the cell migration of Cal27, as determined by wound healing assays. Images of the wound healing assays of the 2 siRNA groups and the negative control group were captured at 0 h, 24 h and 48 h (magnification, × 10). Bar graph indicates the mean healing rate of the 3 experimental repetitions of each group. b Effect of MYOSLID on cell invasion was determined by transwell assay. Representative images of the siRNA group and negative control group were captured after 48 h (magnification, × 20). Bar graph indicates the mean number of cells that passed through the filter coated with Matrigel. Three repeated experiments were performed for each group. c Results of Western blot showed that knockdown MYOSLID in Cal27 cell line caused a significant downregulation of p-EMT related markers Slug, PDPN and LAMB3 protein levels, but protein levels of Classical EMT related markers E-cadherin and Vimentin remain unchanged. “*” indicates that there was statistical significance indicated by a two-sample t test. p < 0.05 was considered statistically significant. The results are presented as the mean ± SEM (n = 3). siRNA-1 refers to MYOSLID-homo-96, and siRNA-2 refers to MYOSLID-homo-323