Table 1.
Antibody information
| Primary antibody name | Company | Catalog # | Monoclonal polyclonal | Species it was raised in | Concentration | Secondary antibody | Catalog # | Company | Concentration | PRID |
|---|---|---|---|---|---|---|---|---|---|---|
| Anti vimentin Produced from pig V9 clone, an IgG isotype; recognizes ~58 kDa protein. |
Sigma | V6389 | Monoclonal | Mouse | 1:200 | Goat antimouse IgG (H + L), Alexa flour 555 | A21422 | Thermo fisher | 1:500 | Sigma‐Aldrich cat# V6389, RRID:AB_609914 |
| Anti AXL Detects total levels of AXL protein and does not cross react with closely related intermediate filament proteins such as desmin & GFAP. |
Cell signaling | C89E7 | Monoclonal | Rabbit | 1:100 | Goat antirabbit IgG (H + L), Alexa flour 488 | A11008 | Thermo fisher | 1:500 | Cell signaling technology cat# 8661S, RRID:AB_11217435 |
| Anti SOX2 Derived from residues ranging from 300 to the C terminus of human Sox2. |
Abcam | ab97959 | Polyclonal | Rabbit | 1:200 | Goat antirabbit IgG (H + L), Alexa flour 488 | A11008 | Thermo fisher | 1:500 | Abcam cat# ab97959, RRID:AB_234119 |
| Anti CD31 Raised within aa 700 to the C‐terminus. |
Abcam | ab28364 | Polyclonal | Rabbit | 1:15 | Goat antirabbit IgG (H + L), Alexa flour 555 | A21428 | Thermo fisher | 1:500 | Abcam cat# ab28364, RRID:AB_726362 |