Skip to main content
. 2019 Mar 15;25(4):610–626. doi: 10.1002/lt.25429

Figure 5.

Figure 5

DAMPs released during MP and graft reperfusion are biologically active and stimulate TLRs. Perfusate samples at the end of MP and end of reperfusion were incubated with TLR reporter cell lines. TLR stimulation produces activation of NF‐κB, which is linked to secretion of alkaline phosphatase into the cell media and detected by a colorimetric assay. (A) TLR3 activation, indicative of binding by double‐stranded RNA. (B) TLR4 activation, indicative of binding by HMGB1. (C) TLR9 activation, indicative of binding by exDNA. Data are shown as mean ± SEM, n = 6 per group. *P < 0.05, **P < 0.01, ***P < 0.001.