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. 2019 Mar 25;49(5):694–708. doi: 10.1002/eji.201847897

Figure 4.

Figure 4

IL‐7RαhiKLRG1lo cells with noncytotoxic memory traits are enriched in lymph nodes. (A) Flow cytometric analysis of the expression of KLRG1 and IL‐7Rα in nonnaive CD8+ T cells in peripheral blood (PB; left) and (right), distribution of KLRG1/IL‐7Rα‐defined subsets in nonnaive CD8+ T cells of 14 PB with paired LN samples (below) and spider plot of mean (right). (B) Spider plot of mean expression of Ki67, CCR7, CD28, CD27, granzyme K, eomes, T‐bet, and granzyme B in nonnaive CD8+ T cells of 14 PB with paired LN samples. (C–G) Representative flow cytometry histograms (top) and frequencies of cells expressing Ki‐67 (C), T‐bet (D), granzyme B (E), CD28 (F), and granzyme K (G) on KLRG1/IL‐7Rα‐defined subsets in nonnaive CD8+ T cells of 14 PB with paired LN samples. (H) Spider plot of the mean expression of markers shown in C‐G on PB (left) and LN (right). Data shown (mean ± SD) are representative of three independent experiments with n = 2, 7, and 5 donors per experiment. Statistical analysis used: Wilcoxon matched‐pairs signed rank test. CCR7, CD27, and Eomes expression can be found in Supporting Information Figure 6.