Figure 4.

miR-373 targeted SRCIN1 expression directly via its 3′UTR. (A) Schematic illustration of the putative miR-373 targeting sequences in the 3′UTR of SRCIN1. Mutant sequences are shown in red type. (B) miR-373 or negative control mimics and WT or Mut luciferase reporters were co-transfected into SK-N-BE(2) and SH-SY5Y cells. The luciferase activity was measured by dual-luciferase reporter assay. (C) Expression of miR-373 in SK-N-BE(2) and SH-SY5Y cells transfected with miR-373 or negative control mimics. qRT-PCR (D) and Western blotting (E) were performed to examine SRCIN1 mRNA and protein expression in cells transfected with miR-373 mimics, negative control mimics, antimiR-373 lentivirus or negative control lentivirus. **p<0.01.

Abbreviations: SRCIN1, SRC kinase signaling inhibitor 1; 3′UTR, 3′untranslated region; WT, wild-type; Mut, mutant; qRT-PCR, quantitative real-time PCR; SD, standard deviation.