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. 2019 Feb 6;222(3):1507–1522. doi: 10.1111/nph.15678

Figure 1.

Figure 1

MiR398b represses the expression of superoxidase dismutase (SOD)X. (a) The construct of fused‐yellow fluorescent protein (YFP) with the target site of SODX (SODXts‐YFP) and alignment of miR398b with SODX ts target site sequences. (b) The construct of fused‐YFP with the mutated target site of SODX (SODXmts‐YFP) and alignment of miR398b with SODX mts target site sequences. The red letters indicate the mutated bases. (c) Alignment of miR398b with target mimicry of miR398 (MIM398) target site sequences. (d, e) Confocal images show the protein concentrations of SODXts‐YFP and SODXmts‐YFP. The indicated SODXts‐YFP and SODXmts‐YFP reporter constructs were transiently expressed alone or coexpressed with miR398b (d) or miR398b plus MIM398 (e) in Nicotiana benthamiana leaves using Agrobacterium‐mediated infiltration at the indicated optical density(OD)concentration. Bars, 50 μm. (f) Western blotting analysis shows the protein concentrations of SODXts‐YFP and SODXmts‐YFP. Protein extracts from the same amount of infiltrated leaves were subjected to Western blot analysis using anti‐green fluorescent protein (GFP) sera and anti‐actin sera, respectively. This experiment was repeated two times with similar results.