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. 2019 Mar 25;26(7):992–999. doi: 10.1111/ene.13922

Figure 1.

Figure 1

Cell‐based assays for Netrin‐1 receptor antibody detection. HEK293T cells transfected with either enhanced green fluorescent protein (EGFP) tagged UNC5A (a) or commercial fluorescent antibody against DCC (b) (left panels). Immunoglobulin G from patients, but not from controls, bind the cells (central panels), co‐localizing with the EGFP tagged UNC5A or with the DCC antibody (merging, right panels; 4′,6‐diamidino‐2‐phenylindole (DAPI) stained nuclei). Scale bar 20 μm. [Color figure can be viewed at wileyonlinelibrary.com].