Figure 4.

MCF7-derived ducts cocultured with adipose stromal cells (ASCs) derived from obese individuals are more resistant to anastrazole treatment. A) MCF7-derived ducts cocultured with lean or obese mammary stromal cells in the presence of 10 nM T were exposed to 5 concentrations of anastrazole for 48 h then evaluated for ER transactivation. Right panel shows individual patients and left panel shows patients merged into a single curve. B) The vehicle-treated (e.g., no anastrazole but exposed to 10 nM of T) cultures of D were lysed and evaluated for ER-driven genes TFF1 and PGR. C) Patient BMI was graphed against their correlating anastrazole IC₅₀. D) MCF7 cells cocultured with lean or obese mammary stromal cells in a 2D well plate in the presence of 10 nM T were exposed to 5 concentrations of anastrazole for 48 h, then evaluated for ER transactivation. Right panel shows individual patients and left panel shows patients merged into a single curve. *P < 0.05 vs. BMI < 25 ASC.