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. 2019 Apr 16;33(7):8335–8348. doi: 10.1096/fj.201801881RR

Figure 1.

Figure 1

Generation of NR2E3 KO mouse using the CRISPR/CAS9 gene editing system. A) Four selected sgRNA sites (flag) in exon 1 and exon 2 are shown. B) CRISPR/CAS9-mediated deletion of 519 bp from mouse NR2E3 exon 1 and exon 2 regions was confirmed by the size difference between 806 bp fragment amplification from WT vs. 287 bp bands from NR2E3−/− KO founder mice after PCR amplification of the target region. C, D) NR2E3 ablation in vivo was confirmed by immunoblotting. Consistently, reduced protein and mRNA levels of previously identified NR2E3 downstream target gene Ahr were detected but not p53 (WT: n = 4, Nr2e3−/− KO: n = 4). Values are represented as means ± sd. **Significant repression; P < 0.05.