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. 2019 Apr 16;33(7):8335–8348. doi: 10.1096/fj.201801881RR

Figure 5.

Figure 5

Effects of NR2E3 ablation in vivo on DINO epigenetic status and chromatin accessibility. A, right) Schematic diagram for mouse DINO gene proximal promoter region containing two consensus Sp1 binding sequences (oval shape). A, left) Effect of NR2E3 loss on the binding status of NR2E3 and Sp1 on the Dino gene promoter proximal region (−213 to +63), as determined by ChIP-PCR assay. B) Re-ChIP analysis of NR2E3–Sp1 complex formation on the DINO proximal promoter region in the presence or absence of NR2E3 in vivo. C) Active histone modification status, including H4Ac, H3K4me2, and H3K4me3. D) The binding status of LSD1, histone deacetylase 1, and repressive histone mark H3K9me3 in the DINO gene promoter proximal region. E) Effects of NR2E3 loss on the chromatin accessibility of DINO gene promoter region. ChACI, II and III are the primer sets used for measuring chromatin accessibility of three different regions of DINO gene promoter. F) The recruitment of p53 and RNA pol II to DINO gene proximal promoter region after APAP treatment (0 and 6 h) in the livers of APAP-treated WT and Nr2e3−/− KO mice. Values are represented as means ± sd. *Significant induction or **repression; P < 0.05.