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. 2019 Apr 10;33(7):8321–8334. doi: 10.1096/fj.201801794R

Figure 6.

Figure 6

HIF-1α inhibits expression of WNT/β-catenin, differentiation factor genes, and myogenic differentiation of WT and MRL/MpJ MDSPCs. A) Quantitative gene expression analysis of untreated and DMOG-treated MRL/MpJ MPCs (left) and MDSPCs cultured in hypoxia (right). B) Levels of differentiation factors in WT and MRL/MpJ MDSPCs after treatment with DMOG in proliferation medium. C) Immunostaining analysis with MyHC-specific antibody and DAPI of myogenic differentiation in untreated (NT) and HIF-1α inhibitor (PX487)–treated MDSPCs. Fusion index is shown in the lower panel. Scale bars, 50 μm. D) Quantitative gene expression analysis for mRNA levels during myogenic differentiation of WT and MRL/MpJ MDSPCs after treatment with PX487. LEF1, lymphoid enhancer binding factor 1; Osx, osterix; Runx2, runt-related transcription factor 2; TCF1- T-cell specific transcription factor 1. Error bars indicate ±SEM from triplicates. *P < 0.01, **P < 0.001.