Figure 1.

The uptake of OATP substrates, ES and TC, are inhibited by UDCA in placental villous fragments. A) ³H-ES uptake by villous fragments was shown to be linear up to 5 min (n = 2 placentas). B) ³H-TC uptake by villous fragments was shown to be linear up to 2.5 min (n = 3 placentas). C) ³H-ES uptake was inhibited by 1 mM ES (***P < 0.001, n = 5), 1 mM TC (***P < 0.001, n = 5), and 1 mM UDCA (**P < 0.01, n = 5) but not by the negative control glycine (n = 5). D) ³H-TC uptake was inhibited by 100 μM UDCA (***P < 0.01, n = 5), 100 μM TC (**P < 0.01, n = 5), and 100 μM ES (***P < 0.01, n = 5), but not by the negative control glycine (n = 5). Data are presented as means and sem and were analyzed by a 1-way ANOVA with Dunnett’s post hoc (compared with ³H-ES and ³H-TC alone).