Figure 6.

A, B) Representative images from immunofluorescence analyses using an antibody against γ-H2AX (MilliporeSigma) in proliferating lymphoblasts from control (A) and MCPH1 cells (B) treated with either DMSO (untreated), ICRF-193, or etoposide. Scale bars, 5 μM. C) Fraction of cells containing more than 5 γ-H2AX foci from the described analyses in A, B. At least 200 cells from 2 independent experiments were counted; mean and range (bars) data are presented. Pooled data for each treatment were compared independently in control and patient cells by χ² test of independence to untreated cells. Furthermore, for each treatment, control and patient data were pairwise compared by χ² test of independence (underlined). Ns, nonsignificant. *P < 0.01, **P < 0.001.