Figure 4.

Expression pattern of POSTN and its splice variants in hCh-1 cells stably transfected clones. To determine whether POSTN and its splice variants are differentially expressed, we compared the expression profile of nontransfected hCh-1 cells (control, n = 3), vehicle cells (vehicle, n = 3), and cells transfected with pcDNA3.1–hPOSTN-001 (hPOSTN-001, n = 3). We observed that POSTN mRNA was significantly higher in control compared with vehicle cells. POSTN isoform 1 was significantly higher on hPOSTN-001 cells compared with control and vehicle cells. Lowercase letters (a) in each graph represent statistical significance from each other at P < 0.05.