TABLE 7.

Validation of RNA-seq data using real-time PCR for selected genes

Gene symbol	Control vs. vehicle				hPOSTN-001 vs. control				hPOSTN-001 vs. vehicle
	RNA-seq		Real-time PCR		RNA-seq		Real-time PCR		RNA-seq		Real-time PCR
	Fold change	P	Fold change	P	Fold change	P	Fold change	P	Fold change	P	Fold change	P
MARCKS	686.06	<0.001	∞	—	−464.80	<0.001	∞	—	n.s.	—	0.00	—
HIST1H3I	299.91	<0.001	1489.74	<0.001	−331.21	<0.001	−1950.95	<0.001	n.s.	—	−1.31	0.128
DMD	292.68	<0.001	185.85	<0.001	−87.37	<0.001	−179.32	<0.001	n.s.	—	1.04	0.943
ZNF714	287.84	<0.001	693.09	<0.001	−107.52	<0.001	−901.34	<0.001	n.s.	—	−1.30	0.531
ZNF91	275.43	<0.001	4.77	<0.001	−74.55	<0.001	−5.11	<0.001	n.s.	—	−1.07	0.475
MAL2	−16.55	<0.001	−13.83	<0.001	n.s.	—	1.76	0.001	−12.60	<0.001	−7.88	<0.001
CNTN1	−17.02	<0.001	−15.52	<0.001	−3.61	<0.001	−3.42	<0.001	−61.46	<0.001	−53.02	<0.001
DLEC1	−18.35	<0.001	−337.95	0.001	2.47	0.002	26.16	0.002	−7.43	<0.001	−12.92	0.001
MPZL2	−25.64	<0.001	∞	—	9.48	<0.001	∞	—	−2.71	<0.001	−6.10	0.001
DACH2	−76.22	<0.001	−77.45	<0.001	9.84	<0.001	6.35	<0.001	−7.75	<0.001	−12.21	<0.001
GJB2	−12.89	<0.001	−10.09	<0.001	60.18	<0.001	65.11	<0.001	4.67	<0.001	6.45	<0.001
LRP1B	−7.45	<0.001	−7.19	<0.001	42.41	<0.001	46.62	<0.001	5.70	<0.001	6.48	<0.001
HSD17B3	n.s.	—	1.11	0.553	36.43	<0.001	−1.04	0.718	44.18	<0.001	1.07	0.690
ZIC3	−5.20	<0.001	−6.64	<0.001	24.05	<0.001	29.75	<0.001	4.63	<0.001	4.48	0.000
PARVA	80.56	<0.001	3044.90	<0.001	−401.67	<0.001	−3490.57	<0.001	−4.99	0.011	−1.15	0.777
HIST1H2BE	108.21	<0.001	−1.63	0.005	−423.59	<0.001	1.18	0.135	n.s.	—	−1.39	0.005
TSPYL5	250.62	<0.001	136,573.66	0.006	−461.22	<0.001	−61,464.31	0.006	n.s.	—	2.22	0.222
PCDHB5	94.91	<0.001	107.79	<0.001	−667.80	<0.001	−120.79	<0.001	−7.04	<0.001	−1.12	0.195
CMKLR1	3.49	0.002	4.49	<0.001	9.17	<0.001	11.63	<0.001	32.04	<0.001	52.22	<0.001
TRPM3	2.84	0.015	−1.51	0.558	8.81	<0.001	17.71	<0.001	25.00	<0.001	11.75	<0.001
DRD2	12.71	<0.001	16.03	<0.001	1.66	0.006	1.50	0.008	21.12	<0.001	24.12	<0.001
OLFM3	n.s.	—	1.27	0.077	−50.24	<0.001	−336.41	<0.001	−41.78	<0.001	−265.75	<0.001
ITGA8	−1.82	<0.001	−1.44	0.005	−35.53	<0.001	−101.14	<0.001	−64.64	<0.001	−145.75	<0.001
LUZP2	−3.57	<0.001	−6.12	<0.001	−19.31	<0.001	−5.42	<0.001	−68.84	<0.001	−33.15	<0.001
RIPPLY3	n.s.	—	−1.13	0.286	−52.75	<0.001	−61.36	<0.001	−70.45	<0.001	−69.33	<0.001

n.s., not significant.