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. 2019 Mar 27;248(5):375–395. doi: 10.1002/dvdy.23

Figure 5.

Figure 5

LPA2 receptor protein expression in mouse brain. A: LPA2 antibody specificity is verified by immunostaining of WT and LPA2 KO mice brains at P15. Representative fluorescence images of LPA2 (green) expression in WT CA1 region of the hippocampus shows punctuated LPA2 protein expression in somata and dendrites of pyramidal CA1 neurons, whereas staining is not detectable in KO mice. B: Neurons were visualized with the specific marker for postmitotic immature neurons Tuj1 (red). LPA2 receptor showed a signal in the extensions of Tuj1‐positive neuroblasts (green, arrows). C: Representative confocal images of LPA2 double‐stained with the astrocyte marker GFAP (red). LPA2 receptor is expressed in the cell soma and in astrocyte processes. D: Representative fluorescence images of LPA2 (green) expression in mouse CA1 hippocampal region and cerebellum at P30 is shown by immunohistochemistry. GL, granular layer; H, hilus; KO, knockout; ML, molecular layer; OR, striatum oriens; P, postnatal day; PY, striatum pyramidale; RAD, striatum radiale; SGZ, subgranular zone; WM, white matter; WT, wild‐type. Scale bars A,D = 20 μm. Scale bar C = 10 μm