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. 2019 Jun 21;11:5557–5572. doi: 10.2147/CMAR.S203060

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© 2019 Xu et al.

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IRF6 expression is elevated in breast cancer tissues and breast cell lines. (A and B) The mRNA expression levels of IRF6 in breast cell lines were determined by RT-qPCR and agarose gel electrophoresis. (C) The protein levels of IRF6 in breast cancer cell lines and non-cancerous breast cell lines were confirmed by immunoblotting analysis; GAPDH was used as a normalized control. (D) The mRNA expression levels of IRF6 in breast cancer tissues and non-cancerous breast tissues were determined by RT-qPCR. Student’s t-test, *p<0.05.