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. Author manuscript; available in PMC: 2019 Jun 26.
Published in final edited form as: ACS Chem Biol. 2017 Oct 11;12(11):2804–2814. doi: 10.1021/acschembio.6b01035

Figure 3.

Figure 3

Upregulation of lysine acetylation of histone H3 N-terminal tail in castrate-resistant C4–2. (A) Lysine acetylation of histone H31–20 peptide by endogenous HAT activity in C4–2 vs LNCaP as monitored by isotopic incorporation of a tritium-labeled acetyl group from 3H-acetyl-CoA shows higher acetylation on the H3 peptide when incubated with C4–2 compared with LNCaP. (B) Quantification of Western blot analyses showing endogenous acetylation level at H3K9, H3K14, and H3K18 sites in LNCaP compared to C4–2. Each histone PTM was normalized against the histone H3 band (n = 3 for each PTM). All data represent the mean ± s.d. *P ≤ 0.05 and **P ≤ 0.01.