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. Author manuscript; available in PMC: 2019 Aug 21.
Published in final edited form as: Immunity. 2018 Jul 24;49(2):275–287.e5. doi: 10.1016/j.immuni.2018.06.009

Figure 4. CSF1-CSF1R pathway promotes alveolar cDC2 migration to regional LNs.

Figure 4.

(A-B) Mass cytometry of human BAL cells before (Pre) and at 48 hours (Post) after allergen challenge. From the cDC gated population, the numbers of cDC1, cDC2 and CSF1R+ cells were calculated. (C) The percentage of CSF1R+ cells among alveolar macrophages (Amac), cDC1 and cDC2 were measured with or without DRA challenges in the BAL fluids at 6, 24 h after last challenge. (D & E) Number of CSF1R+ cDC1 and cDC2 in BAL fluid and mediastinal LNs with DRA challenge or vehicle in Scgb1a1-creERT;Csf1fl/fl with or without Tm injection. (F-G) Fluorophore ZW800-conjugated HDM (ZW-HDM) was measured in the mediastinal LNs in the DRA asthma model. After gating to the CD172a+ cDC2 population, the ZW-HDM+ cells were counted in Scgb1a1-creERT;Csf1fl/fl mice with or without Tm injection in the presence or absence of GW2580 (10 mg/kg, i.p). Data in C-G are at least two of independent experiments. *p<.05, **p<.01. Please also see figure S2–S3.