Figure 1.

Separation of MyHC isoforms by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) for representative bovine muscle samples. Lanes: 1, biceps femoris (proximal portion); 2, biceps femoris (distal portion); 3, rectus femoris; 4, vastus lateralis; 5, semimembranosus; 6, semitendinosus; 7, iliacus; 8, gluteus accessorius; 9, flexor digitorum superficialis; 10, serratus ventralis; 11, serratus cervicis; 12, trapezius; 13, rhomboideus thoracis; 14, latissimus dorsi; 15, longissimus thoracis; 16, spinalis and semispinalis; 17, splenius capitis; 18, subscapularis; 19, infraspinous; 20, external oblique; 21, psoas major; and ref, a mix of rat extensor digitorum longus muscles and soleus sample that was used as the four MyHC isoform reference (migration rate is MyHC1 > 2B > 2X > 2A)