Figure 6.

CD4+ T cells induced chemoresistance of PCa via CCL5/P‐STAT3 signaling. A, B, Effect of P‐STAT3 inhibitor treatment. Cell viability was detected using CCK8, Data are presented as mean ± SD, n = 3. *P < 0.05 vs control. and cell apoptosis was detected by Tunel assay. C4‐2 and 22Rv1 cells were pretreated with S3i‐201 (P‐STAT3 inhibitor) for 24 hours and subsequently coculture with CD4+ T cells for an additional 24 hours, and then treated with 3 nM Docetaxel for 48 hours. The P‐STAT3 inhibitor treatment can reverse CD4+ T cells induced chemoresistance of PCa from CCK8 and Tunel assay results. CCK8, cell counting kit‐8; CCL5, C‐C motif chemokine ligand 5; PCa, prostate cancer; STAT3, signal transducer and activator of transcription 3; Tunel, terminal deoxynucleotidyl transferase dUTP nick‐end labeling [Color figure can be viewed at wileyonlinelibrary.com]