Figure 3.

HTR8/SVneo cells promoted the expression of inhibitory receptors in differentiated Treg cells. Maternal naïve CD4⁺T cells were differentiated in the presence of Th0‐, Th17‐, or Treg‐inducing differentiation agents for 5 days. In some wells, CD4⁺T cells were co‐cultured with HTR8/SVneo cells. Cytotoxic T lymphocyte‐associated protein‐4 (CTLA‐4) (A), Tim‐3 (B), and programmed cell death‐1 (PD‐1) (C) expression in CD4⁺T cells was analyzed through flow cytometry. **P < 0.01, ***P < 0.001, compared to Th0 group. #P < 0.05, ###P < 0.001, compared to Treg group. Data are represented as the mean ± SD, n = 18. Flow cytometry plots are representative of three independent experiments