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. Author manuscript; available in PMC: 2019 Jun 26.
Published in final edited form as: J Vis Exp. 2019 Mar 8;(145):10.3791/59013. doi: 10.3791/59013

Figure 5: Accuracy and reproducibility of ultrasound imaging.

Figure 5:

(A) Representative images of thoracic aortic ultrasound and ex vivo images in C57BL/6J male mice (10–12 weeks old). Bland-Altman plots show (B) inter- and (C) intraobserver variabilities of this protocol. Asc Ao = ascending aorta, IA = innominate artery, LCA = left common carotid artery, LSA = left subclavian artery, PA = pulmonary artery, and Sinus = aortic sinus. The green line indicates the center of the ascending aorta. The yellow and red lines indicate the diameters of the aortic sinus and ascending aorta, respectively. Digits in red colors denote the actual diameters of the ascending aorta measured in ultrasound and ex vivo images. The black dotted lines indicate the mean and mean ± 1.96 SD. Please click here to view a larger version of this figure.