Fig. 6.
TCDD promotes P-RelA/p65 degradation in WT mouse macrophages. Peritoneal macrophages (1 × 106 cells/mL) from WT mice were pretreated with 10 nM TCDD at the indicated times and activated with LPS/IFNγ (1 μg/mL and 20 ng/mL, respectively) for 30 min. Control cultures were activated with LPS/IFNγ. A) P-RelA/p65 and RelA/p65 were determined by Western blot. Representative blots and densitometric scanning of the blots are shown. β-Actin was used as a loading control. The data are presented as the mean ± S.D. of three independent experiments. *p < 0.05, control vs. treatments. B) Representative confocal microscopy images of P-RelA/p65 are shown after macrophage activation only or after 12 h of TCDD pretreatment and LPS/IFNγ. P-RelA/p65, actin, and DAPI were visualized as green, red, and blue, respectively. DMSO was used as a control. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)